EDTA-铜(螯合铜)

chrisxi

螯合铜为一种利用特殊螯合剂的螯合效应于铜元素结合 形成螯合结构的错化合物 此种化合物以铜元素为中心原子 周围被螯合剂的分子包围 形成两个以上的交错位置 结构如同蟹螯 相对于有机铜（醋酸铜 柠檬酸铜）更加稳定  美国在线杂志Advanced Aquarist    Terry D. Bartelme的一篇关于螯合铜（EDTA-铜）的论文  其中有提到海化Seachem  Cupramine白点水（这个小弟最近正在使用 的确非常稳定 检疫缸同时开化蛋 甚至长时间臭氧 铜含量都无减少 ）   ~~~~小弟E文水平实在有限 无法做到全文翻译 还请各位大大加减看啦~~~

Limited number of reliable treatments

There are many purported treatments for cryptocaryonosis. Unfortunately, the majority of these methods or therapies are only partially effective at best. There are currently only three proven, consistently reliable, treatments. These consist of copper treatment, salinity manipulation (i.e. hyposalinity) and the transfer method. Even the best treatments currently available have limitations and/or drawbacks.



Copper treatment

Copper therapy is the most well established means of combating Cryptocaryon irritans (Cardeilhac & Whitaker, 1988). However, copper is lethal to invertebrates, micro fauna and micro flora. Copper is removed from solution by calcium, magnesium and organics.

Copper-based medications (some contain other chemicals in combination with copper) should only be administered in an aquarium that does not contain invertebrates, rock, substrate or other calcareous material.

Copper suppresses immune function and is highly stressful to fish. Copper is also toxic to fish, but to a lesser degree than with invertebrates. Administering a dose that is too high may kill the fish being treated. If the copper level is not high enough then the treatment will be ineffective. This necessitates testing the copper level twice a day and making adjustments as needed.

Take care to read the recommended dosage in the instructions when using any copper-based medication. The correct dosage varies with the product. Test the copper level twice a day with a test kit that will accurately measure the particular type of copper that you are medicating with. The results of using an incorrect dosage with any copper-based medication can be catastrophic. Chelated forms of copper tend to require a higher dose and they are generally less effective than the non-chelated forms. A therapeutic copper level should be maintained continuously for a minimum of three weeks.

I have personally used Cupramine™, a Seachem product, several times with "copper sensitive species" such as lionfish, puffers, dwarf angels and mandarins with impressive results. In my experience, it has been more effective and better tolerated than other forms of copper. Seachem recommends .5ppm as the correct dosage for Cupramine. However, I have used this product successfully at .4ppm.

Hyposalinity therapy

Hyposalinity therapy has numerous advantages over copper-based medications (Bartelme, 2001c). This method does not suppress immune functions such as phagocytic activity. Antibiotics can be used in conjunction with hyposalinity therapy. Some antibiotics are more effective, or a lower dose is required when the salinity is less than that of natural seawater. The salinity only needs to be checked once a day while administrating treatment. Chemical filters such as carbon and Poly Filter™ can be used when employing hyposalinity therapy.

An accurate means for measuring the salinity is crucial when treating fish with hyposalinity. Swing-arm type hydrometers are notoriously inaccurate. A refractometer, or lab grade, large, glass hydrometer is recommended. Alkalinity and pH tend to fall in diluted saltwater. Check these parameters daily and add a buffer as necessary to maintain the pH between 8.1 and 8.3. Do not expose elasmobranches, invertebrates, live rock, or live sand to treatment with hyposalinity. This method is safe for the bacteria that perform biological filtration, at least if the salinity is not dropped too rapidly. Make two water changes per day for two days, reducing the salinity about 5ppt per water change.

Maintaining the salinity at 16ppt or less has proven to be a highly effective treatment for cryptocaryonosis (Bartelme, 2001a, b). However, this may change with if low salinity variants of Cryptocaryon irritans become common or widespread. The salinity (not to be confused with specific gravity) must be maintained consistently at 16ppt or less for the entire duration of treatment. I suggest 14ppt to allow for any fluctuations in the salinity during therapy while providing some margin for error.

Treatment should continue for a minimum of three weeks after a therapeutic salinity level has been reached. Unlike most other forms of treatment for cryptocaryonosis, hyposalinity does not target the "free-swimming" or theront stage. Hyposalinity therapy works by interrupting the life cycle at the tomont stage. Tomonts are destroyed by hyposaline conditions, thus preventing re-infection.

Teleost reef fish appear to adapt well to hyposaline conditions. Hyposalinity was also reported as an effective treatment for cryptocaryonosis by Angelo Colorni of Israel Oceanographic and Limnological Research Ltd 1985 (Colorni, 1985). A report in Drum and Croaker stated: "We now have experience that proves that a wide variety of teleosts can live quite comfortably at ½ salinity (1.010) for extended periods of up to 2 to 3 months (Goodlett & Ichinotsubo, 1997). Emperor angelfish Pomacanthus imperator were the subjects of one such study. They were kept in salinities as low as 7ppt for 30 days without any apparent ill effects (Woo & Chung, 1995).

An alternate method of hyposalinity therapy

Treating fish with a series of short-term baths at a salinity of 8 to 10ppt will effectively destroy tomonts. This method requires a three hour treatment every third day for a total of four treatments. All tomonts exposed to a salinity of 10ppt for three hours eventually degenerated (Colorni, 1985). In the event of an infection with a low salinity variant of Cryptocaryon irritans, such as the Chiayi isolate or strain, a salinity of 8 to 10ppt will not effectively eradicate the parasite.

Handling fish every third day that are already weakened by disease may be risky (Colorni, 1985). Teleost reef fish have an internal salinity of 11 to 12ppt. It is questionable as to whether many species of teleost reef fish that can tolerate an external salinity level below that found in their internal fluids without undue stress. Theoretically, the vectors of ionic and osmotic regulatory processes must be reversed as a saltwater fish crosses the line of equal molarity (Evans, 1984). However, recent studies indicate that at least some species of marine teleost fish are capable of adapting to salinities lower than those found in their internal fluids (Woo & Chung, 1995).



Transfer method

The transfer method consists of moving the fish every third day for a total of four moves between two aquariums. The aquariums are then dried between uses to kill the tomonts that are left behind (Noga, 2000. Colorni, 1985). This will effectively interrupt the life cycle of this parasite. Again however, the stress of frequent handling and the potential injuries associated with such a practice could threaten the health and well being of the fish in question (Noga, 2000).

wppwhy

看天书...

猪八戒

螯合剂配铜离子行吗？或者干脆用捷宝的泵

chrisxi

化学名称:
   乙二胺四乙酸铜钠
EDTA-CuNa2（CAS-No:14025-15-1）
  分子式:
   C10H12N2O8CuNa2
  分子量:
   397.7
外观: 蓝色粉末
铜含量: 15.0±0.5%
pH值（1%水溶液）：6.5±0.5
  主要性能：
   EDTA-Cu-15是一种稳定的水溶性金属螯合物，其中铜以螯合态存在。
水溶性：约 1200g/1（20 ℃）

chrisxi

Experimental treatments

The search for new chemicals and methods to control Cryptocaryon irritans continues. The following two treatments are presented as examples and are not intended as a comprehensive list.

The effectiveness of fatty acids against Cryptocaryon irritans was examined using the red sea bream Pagrus major (Hirazawa, et al., 2001). Caprylic acid was found to have the strongest antiparasitic effect. The fish were fed caprylic acid at a rate of 75mg/kg of body weight per day. The results indicated that caprylic acid has an antiparasitic effect against Cryptocaryon irritans, reducing the number trophonts on the fish. However, despite the reduced number of Cryptocaryon irritans, all of the test subjects died as a result of the infection.

The Food and Drug Administration (FDA) has classified hydrogen peroxide as a Low Regulatory Priority (LRP) drug for use in controlling fungus on fish and fish eggs. Hydrogen peroxide has been experimentally proven to be effective against Amyloodinium sp., a marine fish ectoparasite. It was used at a dosage of 25ppm for 30 minutes to treat Pacific Threadfin, Polydactylus sexfilis infected with Amyloodinium ocellatum. (Montgomery et al., 1999b). Some species of fish tolerate the treatment well, but others are highly sensitive to the chemical (Noga, 2000). Results may also vary between juvenile and adult fish.

Hydrogen peroxide is effective against other ectoparasites, such as Ambiphrya and Gyrodactylus spp. (Rach et al. 2000). Sodium percarbonate is a compound that releases hydrogen peroxide when dissolved in water. Sodium percarbonate was demonstrated to kill the freshwater ectoparasite, Ichthyophthirius multifiliis, at the theront stage or free-swimming, infective stage (Buchmann, et al., 2002). It is currently used in Denmark with rainbow trout Oncorhynchus mykiss at a concentration of 50-100 mg/L is twice a week without any apparent ill effects on the fish.

If more than 50% of the theronts died the concentration of hydrogen peroxide was recorded as effective (Buchmann, et al., 2002). A dosage of 12.5mg/L at a temperature of 12°C kills theronts within 3 hours. This same dosage was not effective against the tomocysts stage of Ichthyophthirius multifiliis. However, dosages of 12.5 mg/L for 180 min and 62.5 mg/L for 90 min were effective against theronts (Buchmann, et al., 2002).

The life cycle of Ichthyophthirius multifiliis is temperature dependant, so the warmer the water temperature the shorter the duration of the parasites life cycle. At 12°C, the medium time frame for tomocsyts to hatch is 9 days and the attached parasitic stage has a duration of 10-12 days. This means that at 12°C treatment should continue daily for a minimum of three weeks. Caution should be taken when using hydrogen peroxide as accidental spills may have an immediate adverse effect on human skin.

Studies to test the effectiveness and safety of hydrogen peroxide for the treatment of other ectoparasites such as Cryptocaryon irritans are fully warranted (Montgomery-Brock, D. et al., 2000). However, this treatment is considered to be highly experimental, therefore it cannot be recommended. The side effects and survival rate when using hydrogen peroxide may not prove to be acceptable. Protective clothing and safety glasses should be worn when using a dose of 35% and higher. The water temperature should be carefully monitored when treating with hydrogen peroxide, because it becomes more toxic as the temperature rises. At this point, the safety, effectiveness, correct dosage and duration of treatment for this experimental method have not been established.



To be continued

The forth installment in this five part series will cover some alternative methods of combating Cryptocaryon irritans. These will include the use of formaldehyde and malachite green, ultra violet sterilizers, freshwater dips, hypersalinity and the sand removal method. Part four will also cover the use of biological cleaners, antimalaria drugs, raising the water temperature, the so-called "reef safe medications and the use of herbal remedies (i.e. garlic) to control Cryptocaryon irritans.



References

Bartelme, T.D. "Cryptocaryon irritans: An Update on the Scourge of Marine Aquariums, Part One." Freshwater and Marine Aquarium Magazine, February 2001a.

Bartelme, T.D. "Cryptocaryon irritans: An Update on the Scourge of Marine Aquariums, Part Two." Freshwater and Marine Aquarium Magazine, March 2001b.

Bartelme, T. D. "Treating Saltwater Ich without Medication" Tropical Fish Hobbyist, January 2001c.

Buchmann, K. Jensen, P. B. & Kruse, K. D. "Effects of Sodium Percarbonate and Garlic Extract on Ichthyophthirius multifiliis Theronts and Tomocysts: In Vitro Experiments." Department of Veterinary Microbiology, Section of Fish Diseases, Royal Veterinary and Agricultural University, Stigbøjlen 4, DK-1870 Frederiksberg C., Denmark, 2002.

Cardeilhac, P. & Whitaker, B. "Copper Treatments: Uses and Precautions." Veterinary Clinics of North America: Small Animal Practice, 18, 85-88, 1988.

Cheung, P.J., Nigrelli, R.F. & Ruggieri, G.D. "Studies on Cryptocaryonosis in Marine Fish: Effect of Temperature and Salinity on the Reproductive cycle of Cryptocaryon irritans Brown 1951." Journal of Fish Diseases, 2, 93-97, 1979.

Colorni, A. "Aspects of the Biology of Cryptocaryon irritans and Hyposalinity as a Control Measure in Cultured Gilt-Head Sea Bream Sparus aurata." Diseases of Aquatic Organisms. 1, 19-22, 1985.


Evans, D.H. "The Roles of Gill Permeability and Transport Mechanisms in Euryhalinity." Fish Physiology, 10, part B (Hoar, W.S. & Randall, D.J., Eds), pp. 239-283. New York Academic Press. 1984.

Goodlett, R. & Ichinotsubo, L. "Salinity and pH Adjustments for Quarantine Procedures for Marine Teleost Fishes." Drum and Croaker, 28, 23-26, January 1997.

http://www.colszoo.org/internal/drum_croaker/pdf/1997.pdf

Hirazawa, N. Oshima, S-I. Hara, T. Mitsuboshi, T. & Hata, K. "Antiparasitic Effect of Medium-Chain Fatty Acids against the Ciliate Cryptocaryon irritans Infestation in the Red Sea Bream Pagrus major." Aquaculture, 198(3-4), 219-228, 2001.


Montgomery-Brock, D. Sylvester, J.Y. Tamaru, C. S. & Brock, J. "Hydrogen peroxide treatment for Amyloodinium sp. on mullet ( Mugil cephalus) fry." Regional Notes, 11(4), Summer 2000.

http://www.ctsa.org/upload/note/RN_11_4631705490300293193.pdf

Montgomery, D., J. Brock and V.T. Sato. "Using hydrogen peroxide for Pacific threadfin infected by Amyloodinium ocellatum." Regional Notes, 10(2), Winter 1999b.

Noga, E.J. "Fish Disease: Diagnosis and Treatment." Ames, IA: Iowa State University Press, 2000.

Rach, J.J. Gaikowski, M.P. & Ramsay, R.T. "Efficacy of Hydrogen Peroxide to Control Parasitic Infestations on Hatchery-Reared Fish." Journal of Aquatic Animal Health, 12, 267-273, 2000.

Copyrights: Terry D. Bartelme

chrisxi

海化白点水 似乎是比螯合更加先进的络合铜哦

chrisxi

原帖由 猪八戒 于 2009-3-22 08:51 发表
螯合剂配铜离子行吗？或者干脆用捷宝的泵

不用自己配啦 乙二胺四乙酸铜钠（EDTA.CuNa2 EDTA铜钠盐） 化工店应该买得到 这东西用于农业微量元素营养剂 不过好像都是25KG大包装的 买一袋三辈子都用不完

f999

我买了三瓶，检疫的时候用。

龙马

螯合铜没试过。
我用乙二胺四乙酸钠+硫酸铁，调出来的螯合铁在淡水水草缸用得很好，进了海水藻缸基本是没效，还是要专用的海水铁肥。

ciboys

没有看明白

wgfhp

一起交流！对这个话题感兴趣的朋友们












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vzx520

非常棒 我试着翻译了一下

http://www.cmfish.com/bbs/viewthread.php?tid=159705&extra=

我贴到这里来了

cuihaowei

弥生水奈

海化检疫确实不错，很安全，配合铜测试

汤米.cc

高学问。。。

monkey_pig

文化贴，作为屌丝的我看不懂。。。

低調的上帝

过江龙

好在的土豪缸，，，，

安于宿命。

帮顶

lyq0504

顶一下，一点没看懂

飞车